On the Observation of Wild Zebrafish (Danio rerio) in India.

On the Observation of Wild Zebrafish (Danio rerio) in India.

Zebrafish is without doubt one of the world’s most generally used laboratory species, and it’s utilized to reply vital analysis questions in disparate fields reminiscent of biomedicine, genetics, developmental biology, pharmacology, toxicology, physiology, and evolution. Regardless of their reputation, little or no is understood concerning the biology of zebrafish of their pure habitat.

This may increasingly, partially, be because of the difficulties related to endeavor area journeys to the distant areas of northern India, Nepal, and Bangladesh, which is the pure distribution vary of zebrafish. Right here, we current a area report describing a latest journey the place we, along with native collaborators, visited a number of rivers in West Bengal, India, to watch wild zebrafish and their habitat. We current an outline of our observations on the biology of untamed zebrafish, and the nice variability of the totally different environments the place they have been discovered.

We additionally embrace knowledge collected on water chemistry parameters at 12 zebrafish websites, and weight knowledge and pictures of fish from these websites. We current in depth underwater movies of untamed zebrafish and images of the websites, together with video footage of courtship conduct. We present that the breeding interval of untamed zebrafish could be prolonged from the earlier file of April-August to April-October. As well as, we offer sensible recommendation for future zebrafish expeditions to this rural and inaccessible space. The objectives of this text are to shed some mild on the ecology of untamed zebrafish, and to facilitate scientists of their future analysis journeys. We hope that by observing zebrafish within the wild, we are able to enhance our understanding of the pure ecology of this vital mannequin organism.

Use of Zebrafish in Drug Discovery Toxicology.

Unpredicted human security occasions in scientific trials for brand new medicine are expensive when it comes to human well being and cash. The drug discovery business makes an attempt to attenuate these occasions with diligent preclinical security testing. Present normal practices are good at stopping poisonous compounds from being examined within the clinic; nonetheless, false unfavorable preclinical toxicity outcomes are nonetheless a actuality. Continuous enchancment have to be pursued within the preclinical realm. Increased high quality therapies could be introduced ahead with extra details about potential toxicities and related mechanisms.
The zebrafish mannequin is a bridge between in vitro assays and mammalian in vivo research. This mannequin is highly effective in its breadth of utility and tractability for analysis. Prior to now 20 years our understanding of illness biology and drug toxicity has grown considerably owing to hundreds of research on this tiny vertebrate. This overview summarizes challenges and strengths of the mannequin, discusses the 3Rs worth that it could ship, highlights translatable and untranslatable biology, and brings collectively experiences from latest research with zebrafish specializing in new drug discovery toxicology.
The priority about DNA injury has directed efforts towards evaluating the genotoxic potential of bodily and chemical brokers. For the reason that extent of DNA injury can be associated to the capability of the organism in repairing the DNA, the advance of toxicological research on this space depends upon the characterization of the DNA restore mechanisms within the accessible fashions. The mobile zebrafish fashions, for instance, exchange mammalian cells to reply ecologically related questions on aquatic toxicology.
So, the intention of the current research was to characterize the nucleotide excision restore (NER) and photoreactivation (PER) in two mobile fashions of Danio rerio liver, major hepatocytes and ZF-L (Zebrafish Liver) cell line. We carried out kinetic research of the DNA injury ranges after publicity to six.eight J/m2 UVC utilizing the T4-PDG modified Comet Assay, and decided the expression ranges of vital genes concerned in NER, PER and base excision restore utilizing RT-qPCR.
On the Observation of Wild Zebrafish (Danio rerio) in India.

Emergence of constant intra-individual locomotor patterns throughout zebrafish growth.

The evaluation of larval zebrafish locomotor conduct has emerged as a strong indicator of perturbations within the nervous system and is utilized in many fields of analysis, together with neuroscience, toxicology and drug discovery. The conduct of larval zebrafish nonetheless, is extremely variable, leading to the usage of giant numbers of animals and the lack to detect small results.
On this research, we analyzed whether or not particular person locomotor conduct is secure over growth and whether or not behavioral parameters correlate with physiological and morphological options, with the intention of higher understanding the variability and predictability of larval locomotor conduct. Our outcomes reveal that locomotor exercise of a person larva stays constant all through a given day and is predictable all through larval growth, particularly throughout darkish phases, below which larvae display light-searching behaviors and elevated exercise.

ak2 Antibody (Zebrafish)

F54506-0.2ML 0.2 ml
EUR 322.15
Description: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.

Ada Antibody (Zebrafish)

F52652-0.08ML 0.08 ml
EUR 140.25
Description: Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation (By similarity).

Zebrafish Ada Antibody

F52652-0.4ML 0.4 ml
EUR 322.15
Description: Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation (By similarity).

Zebrafish LC3A Antibody

F53024-0.08ML 0.08 ml
EUR 140.25
Description: Microtubule-associated protein 1 light chain 3 alpha

Zebrafish LC3A Antibody

F53024-0.4ML 0.4 ml
EUR 322.15
Description: Microtubule-associated protein 1 light chain 3 alpha

Atg5 Antibody (Zebrafish)

F53143-0.08ML 0.08 ml
EUR 140.25
Description: Atg5 is involved in autophagic vesicle formation.

Zebrafish LC3B Antibody

F53289-0.08ML 0.08 ml
EUR 140.25
Description: N/A

Zebrafish LC3B Antibody

F53289-0.4ML 0.4 ml
EUR 322.15
Description: N/A

Atg5 Antibody (Zebrafish)

F53381-0.08ML 0.08 ml
EUR 140.25
Description: Involved in autophagic vesicle formation.

Atg5 Antibody (Zebrafish)

F53381-0.4ML 0.4 ml
EUR 322.15
Description: Involved in autophagic vesicle formation.

Zebrafish Atf4 Antibody

F52258-0.08ML 0.08 ml
EUR 140.25
Description: Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters (By similarity).

Zebrafish Atf4 Antibody

F52258-0.4ML 0.4 ml
EUR 322.15
Description: Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters (By similarity).

Zebrafish Thrb Antibody

R33481-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Thyroid hormone receptor beta

Zebrafish Pax2a Antibody

F52269-0.08ML 0.08 ml
EUR 140.25
Description: Probable transcription factor involved in the development of the midbrain/hindbrain boundary (MHB) organizer and specification of neuronal cell fates. Required for establishment of eng2 and eng3 gene expression in the midbrain and MHB primordium during late gastrulation.

Zebrafish Pax2a Antibody

F52269-0.4ML 0.4 ml
EUR 322.15
Description: Probable transcription factor involved in the development of the midbrain/hindbrain boundary (MHB) organizer and specification of neuronal cell fates. Required for establishment of eng2 and eng3 gene expression in the midbrain and MHB primordium during late gastrulation.

S1pr1 Antibody (Zebrafish)

F52393-0.08ML 0.08 ml
EUR 140.25
Description: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.

Gapdh Antibody (Zebrafish)

F52573-0.08ML 0.08 ml
EUR 140.25
Description: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).

Zebrafish Gapdh Antibody

F52573-0.4ML 0.4 ml
EUR 322.15
Description: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).

Pou3f3a Antibody (Zebrafish)

F52241-0.08ML 0.08 ml
EUR 140.25
Description: Transcription factor that may play important roles in patterning the embryonic brain.

Pou3f3a Antibody (Zebrafish)

F52241-0.4ML 0.4 ml
EUR 322.15
Description: Transcription factor that may play important roles in patterning the embryonic brain.

Zebrafish Patched Antibody

R35480-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Zebrafish patched 1, ptc1; ptc1

Zebrafish Map1lc3a Antibody

F53023-0.08ML 0.08 ml
EUR 140.25
Description: Microtubule-associated protein 1 light chain 3 alpha

Zebrafish Map1lc3a Antibody

F53023-0.4ML 0.4 ml
EUR 322.15
Description: Microtubule-associated protein 1 light chain 3 alpha

Gabarapa Antibody (Zebrafish)

F53276-0.08ML 0.08 ml
EUR 140.25
Description: GABA(A) receptor associated protein

Beclin-1 Antibody (Zebrafish)

F53282-0.08ML 0.08 ml
EUR 140.25
Description: Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.

Zebrafish Beclin-1 Antibody

F53282-0.4ML 0.4 ml
EUR 322.15
Description: Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.

Zebrafish beta Actin Antibody

F52293-0.08ML 0.08 ml
EUR 140.25
Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Expression of beta Actin is strongest in lymph tissues with weaker expression seen in muscle and heart.

Zebrafish beta Actin Antibody

F52293-0.4ML 0.4 ml
EUR 322.15
Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Expression of beta Actin is strongest in lymph tissues with weaker expression seen in muscle and heart.

Zebrafish ak2 Antibody (Center)

MBS9215641-005mL 0.05mL
EUR 210

Zebrafish ak2 Antibody (Center)

MBS9215641-02mL 0.2mL
EUR 430

Zebrafish ak2 Antibody (Center)

MBS9215641-5x02mL 5x0.2mL
EUR 1910

Anti-Thrb (zebrafish) antibody

STJ71268 100 µg
EUR 430.8

Anti-Her6 (zebrafish) antibody

STJ71294 100 µg
EUR 312

Pou5f1 Antibody OCT4 (Zebrafish)

F52574-0.08ML 0.08 ml
EUR 140.25
Description: Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.

Anti-Hand2 (zebrafish) antibody

STJ71256 100 µg
EUR 312

Anti-Six3b (zebrafish) antibody

STJ71263 100 µg
EUR 312

Anti-Sox9a (zebrafish) antibody

STJ71264 100 µg
EUR 312

Anti-Sox9b (zebrafish) antibody

STJ71265 100 µg
EUR 312

Anti-Timp2 (zebrafish) antibody

STJ71270 100 µg
EUR 312

Anti-Atoh7 (zebrafish) antibody

STJ71272 100 µg
EUR 312

Anti-Ptf1a (zebrafish) antibody

STJ71274 100 µg
EUR 312

Zebrafish wnt8b Antibody (Center)

MBS9215866-005mL 0.05mL
EUR 210

Zebrafish wnt8b Antibody (Center)

MBS9215866-02mL 0.2mL
EUR 430

Zebrafish wnt8b Antibody (Center)

MBS9215866-5x02mL 5x0.2mL
EUR 1910

Zebrafish wnt8a Antibody (Center)

MBS9216179-005mL 0.05mL
EUR 210

Zebrafish wnt8a Antibody (Center)

MBS9216179-02mL 0.2mL
EUR 430

Zebrafish wnt8a Antibody (Center)

MBS9216179-5x02mL 5x0.2mL
EUR 1910

Zebrafish PDHA1 Antibody (Center)

MBS9217075-008mL 0.08mL
EUR 210

Zebrafish PDHA1 Antibody (Center)

MBS9217075-04mL 0.4mL
EUR 430

Zebrafish PDHA1 Antibody (Center)

MBS9217075-5x04mL 5x0.4mL
EUR 1910

Anti-Tfap2a (zebrafish) antibody

STJ71254 100 µg
EUR 312

Anti-Ascl1a (zebrafish) antibody

STJ71255 100 µg
EUR 312

Anti-Timp2L (zebrafish) antibody

STJ71271 100 µg
EUR 312

Anti-Cxcr4b (zebrafish) antibody

STJ71293 100 µg
EUR 312

Zebrafish pou5f1 Antibody (Center)

MBS9215662-005mL 0.05mL
EUR 210

Zebrafish pou5f1 Antibody (Center)

MBS9215662-02mL 0.2mL
EUR 430

Zebrafish pou5f1 Antibody (Center)

MBS9215662-5x02mL 5x0.2mL
EUR 1910

Zebrafish pou5f1 Antibody (Center)

MBS9216066-005mL 0.05mL
EUR 210

Zebrafish pou5f1 Antibody (Center)

MBS9216066-02mL 0.2mL
EUR 430

Zebrafish pou5f1 Antibody (Center)

MBS9216066-5x02mL 5x0.2mL
EUR 1910

Anti-Notch1a (zebrafish) antibody

STJ71260 100 µg
EUR 312

Anti-Notch1b (zebrafish) antibody

STJ71261 100 µg
EUR 312

Zebrafish H2A Polyclonal Antibody

E-AB-40557-100uL 100uL
EUR 230
Description: Unconjugated

Zebrafish H2A Polyclonal Antibody

E-AB-40557-200uL 200uL
EUR 380
Description: Unconjugated

Zebrafish H2A Polyclonal Antibody

E-AB-40557-20uL 20uL
EUR 65
Description: Unconjugated

Zebrafish H2A Polyclonal Antibody

E-AB-40557-50uL 50uL
EUR 125
Description: Unconjugated

Zebrafish H2A Polyclonal Antibody

MBS2570450-002mL 0.02mL
EUR 125

Zebrafish H2A Polyclonal Antibody

MBS2570450-005mL 0.05mL
EUR 165

Zebrafish H2A Polyclonal Antibody

MBS2570450-01mL 0.1mL
EUR 230

Zebrafish H2A Polyclonal Antibody

MBS2570450-02mL 0.2mL
EUR 325

Zebrafish H2A Polyclonal Antibody

MBS2570450-5x02mL 5x0.2mL
EUR 1445

Zebrafish ak2 Antibody (N-term)

MBS9232174-01mL 0.1mL
EUR 435

Zebrafish ak2 Antibody (N-term)

MBS9232174-5x01mL 5x0.1mL
EUR 1625

Zebrafish ak2 Antibody (N-term)

MBS9216121-005mL 0.05mL
EUR 210

Zebrafish ak2 Antibody (N-term)

MBS9216121-02mL 0.2mL
EUR 430

Zebrafish ak2 Antibody (N-term)

MBS9216121-5x02mL 5x0.2mL
EUR 1910

Nicalin Homolog (Zebrafish) Antibody

20-abx114104
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  • 150 ul
  • 50 ul

Nicalin Homolog (Zebrafish) Antibody

abx114104-100l 100 µl
EUR 612.5

Zebrafish SIM1 Antibody (N-term)

MBS9231229-008mL 0.08mL
EUR 210

Zebrafish SIM1 Antibody (N-term)

MBS9231229-04mL 0.4mL
EUR 430

Zebrafish SIM1 Antibody (N-term)

MBS9231229-5x04mL 5x0.4mL
EUR 1910

Zebrafish stil Antibody (C-term)

MBS9215833-005mL 0.05mL
EUR 210

Zebrafish stil Antibody (C-term)

MBS9215833-02mL 0.2mL
EUR 430

Zebrafish stil Antibody (C-term)

MBS9215833-5x02mL 5x0.2mL
EUR 1910

Zebrafish hey2 Antibody (C-term)

MBS9216635-005mL 0.05mL
EUR 210

Zebrafish hey2 Antibody (C-term)

MBS9216635-02mL 0.2mL
EUR 430

Zebrafish hey2 Antibody (C-term)

MBS9216635-5x02mL 5x0.2mL
EUR 1910

Zebrafish Pax9 Antibody (N-term)

MBS9217074-008mL 0.08mL
EUR 210

Zebrafish Pax9 Antibody (N-term)

MBS9217074-04mL 0.4mL
EUR 430

Zebrafish Pax9 Antibody (N-term)

MBS9217074-5x04mL 5x0.4mL
EUR 1910

Zebrafish EIF2S1 Antibody (N-term)

MBS9232355-008mL 0.08mL
EUR 210

Zebrafish EIF2S1 Antibody (N-term)

MBS9232355-04mL 0.4mL
EUR 430

Zebrafish EIF2S1 Antibody (N-term)

MBS9232355-5x04mL 5x0.4mL
EUR 1910

Zebrafish Map2k4 Antibody (C-term)

MBS9232356-008mL 0.08mL
EUR 210

Zebrafish Map2k4 Antibody (C-term)

MBS9232356-04mL 0.4mL
EUR 430

Zebrafish Map2k4 Antibody (C-term)

MBS9232356-5x04mL 5x0.4mL
EUR 1910

Zebrafish Tead1b Antibody (C-term)

MBS9231230-008mL 0.08mL
EUR 210

Zebrafish Tead1b Antibody (C-term)

MBS9231230-04mL 0.4mL
EUR 430

Zebrafish Tead1b Antibody (C-term)

MBS9231230-5x04mL 5x0.4mL
EUR 1910

Goat anti-Thrb (zebrafish) Antibody

dAP-1197 50ug
EUR 264.6

Goat anti-Her6 (zebrafish) Antibody

dAP-1204 50ug
EUR 264.6

Goat anti-Her6 (zebrafish) Antibody

MBS420166-01mg 0.1mg
EUR 275

Goat anti-Her6 (zebrafish) Antibody

MBS420166-5x01mg 5x0.1mg
EUR 1095

Goat anti-Thrb (zebrafish) Antibody

MBS420704-01mg 0.1mg
EUR 400

Goat anti-Thrb (zebrafish) Antibody

MBS420704-5x01mg 5x0.1mg
EUR 1660

Zebrafish PPP1R3G Polyclonal Antibody

E-AB-40563-100uL 100uL
EUR 230
Description: Unconjugated

Zebrafish PPP1R3G Polyclonal Antibody

E-AB-40563-200uL 200uL
EUR 380
Description: Unconjugated

Zebrafish PPP1R3G Polyclonal Antibody

E-AB-40563-20uL 20uL
EUR 65
Description: Unconjugated

Zebrafish PPP1R3G Polyclonal Antibody

E-AB-40563-50uL 50uL
EUR 125
Description: Unconjugated

Zebrafish PPP1R3G Polyclonal Antibody

MBS2570452-002mL 0.02mL
EUR 125

Zebrafish PPP1R3G Polyclonal Antibody

MBS2570452-005mL 0.05mL
EUR 165

Zebrafish PPP1R3G Polyclonal Antibody

MBS2570452-01mL 0.1mL
EUR 230

Zebrafish PPP1R3G Polyclonal Antibody

MBS2570452-02mL 0.2mL
EUR 325

Zebrafish PPP1R3G Polyclonal Antibody

MBS2570452-5x02mL 5x0.2mL
EUR 1445

Anti-Patched 1 (zebrafish) antibody

STJ71262 100 µg
EUR 430.8

Goat anti-Sox9a (zebrafish) Antibody

dAP-1191 50ug
EUR 264.6

Goat anti-Hand2 (zebrafish) Antibody

dAP-1193 50ug
EUR 264.6

Goat anti-Timp2 (zebrafish) Antibody

dAP-1196 50ug
EUR 264.6

Goat anti-Ptf1a (zebrafish) Antibody

dAP-1198 50ug
EUR 264.6

Goat anti-Sox9b (zebrafish) Antibody

dAP-1200 50ug
EUR 264.6

Goat anti-Atoh7 (zebrafish) Antibody

dAP-1202 50ug
EUR 264.6

Goat anti-Six3b (zebrafish) Antibody

dAP-1207 50ug
EUR 264.6

Goat anti-Six3b (zebrafish) Antibody

MBS420234-01mg 0.1mg
EUR 275

Goat anti-Six3b (zebrafish) Antibody

MBS420234-5x01mg 5x0.1mg
EUR 1095

Goat anti-Ptf1a (zebrafish) Antibody

MBS420317-01mg 0.1mg
EUR 275

Goat anti-Ptf1a (zebrafish) Antibody

MBS420317-5x01mg 5x0.1mg
EUR 1095

Goat anti-Hand2 (zebrafish) Antibody

MBS420492-01mg 0.1mg
EUR 275

Goat anti-Hand2 (zebrafish) Antibody

MBS420492-5x01mg 5x0.1mg
EUR 1095

Goat anti-Timp2 (zebrafish) Antibody

MBS421441-01mg 0.1mg
EUR 275

Goat anti-Timp2 (zebrafish) Antibody

MBS421441-5x01mg 5x0.1mg
EUR 1095

Goat anti-Sox9a (zebrafish) Antibody

MBS421688-01mg 0.1mg
EUR 275

Goat anti-Sox9a (zebrafish) Antibody

MBS421688-5x01mg 5x0.1mg
EUR 1095

Goat anti-Sox9b (zebrafish) Antibody

MBS421972-01mg 0.1mg
EUR 275

Goat anti-Sox9b (zebrafish) Antibody

MBS421972-5x01mg 5x0.1mg
EUR 1095
The larvae’s response to startle-stimuli was discovered to be unpredictable, with no correlation discovered between response power and locomotor exercise. Moreover, locomotor exercise was not related to physiological or morphological options of a larva (resting coronary heart fee, physique size, dimension of the swim bladder). Total, our findings spotlight the areas of intra-individual consistency, which could possibly be used to enhance the sensitivity of assays utilizing zebrafish locomotor exercise as an endpoint.

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