Zebrafish is without doubt one of the world’s most generally used laboratory species, and it’s utilized to reply vital analysis questions in disparate fields reminiscent of biomedicine, genetics, developmental biology, pharmacology, toxicology, physiology, and evolution. Regardless of their reputation, little or no is understood concerning the biology of zebrafish of their pure habitat.
This may increasingly, partially, be because of the difficulties related to endeavor area journeys to the distant areas of northern India, Nepal, and Bangladesh, which is the pure distribution vary of zebrafish. Right here, we current a area report describing a latest journey the place we, along with native collaborators, visited a number of rivers in West Bengal, India, to watch wild zebrafish and their habitat. We current an outline of our observations on the biology of untamed zebrafish, and the nice variability of the totally different environments the place they have been discovered.
We additionally embrace knowledge collected on water chemistry parameters at 12 zebrafish websites, and weight knowledge and pictures of fish from these websites. We current in depth underwater movies of untamed zebrafish and images of the websites, together with video footage of courtship conduct. We present that the breeding interval of untamed zebrafish could be prolonged from the earlier file of April-August to April-October. As well as, we offer sensible recommendation for future zebrafish expeditions to this rural and inaccessible space. The objectives of this text are to shed some mild on the ecology of untamed zebrafish, and to facilitate scientists of their future analysis journeys. We hope that by observing zebrafish within the wild, we are able to enhance our understanding of the pure ecology of this vital mannequin organism.
Use of Zebrafish in Drug Discovery Toxicology.
Unpredicted human security occasions in scientific trials for brand new medicine are expensive when it comes to human well being and cash. The drug discovery business makes an attempt to attenuate these occasions with diligent preclinical security testing. Present normal practices are good at stopping poisonous compounds from being examined within the clinic; nonetheless, false unfavorable preclinical toxicity outcomes are nonetheless a actuality. Continuous enchancment have to be pursued within the preclinical realm. Increased high quality therapies could be introduced ahead with extra details about potential toxicities and related mechanisms.
The zebrafish mannequin is a bridge between in vitro assays and mammalian in vivo research. This mannequin is highly effective in its breadth of utility and tractability for analysis. Prior to now 20 years our understanding of illness biology and drug toxicity has grown considerably owing to hundreds of research on this tiny vertebrate. This overview summarizes challenges and strengths of the mannequin, discusses the 3Rs worth that it could ship, highlights translatable and untranslatable biology, and brings collectively experiences from latest research with zebrafish specializing in new drug discovery toxicology.
The priority about DNA injury has directed efforts towards evaluating the genotoxic potential of bodily and chemical brokers. For the reason that extent of DNA injury can be associated to the capability of the organism in repairing the DNA, the advance of toxicological research on this space depends upon the characterization of the DNA restore mechanisms within the accessible fashions. The mobile zebrafish fashions, for instance, exchange mammalian cells to reply ecologically related questions on aquatic toxicology.
So, the intention of the current research was to characterize the nucleotide excision restore (NER) and photoreactivation (PER) in two mobile fashions of Danio rerio liver, major hepatocytes and ZF-L (Zebrafish Liver) cell line. We carried out kinetic research of the DNA injury ranges after publicity to six.eight J/m2 UVC utilizing the T4-PDG modified Comet Assay, and decided the expression ranges of vital genes concerned in NER, PER and base excision restore utilizing RT-qPCR.
Emergence of constant intra-individual locomotor patterns throughout zebrafish growth.
The evaluation of larval zebrafish locomotor conduct has emerged as a strong indicator of perturbations within the nervous system and is utilized in many fields of analysis, together with neuroscience, toxicology and drug discovery. The conduct of larval zebrafish nonetheless, is extremely variable, leading to the usage of giant numbers of animals and the lack to detect small results.
On this research, we analyzed whether or not particular person locomotor conduct is secure over growth and whether or not behavioral parameters correlate with physiological and morphological options, with the intention of higher understanding the variability and predictability of larval locomotor conduct. Our outcomes reveal that locomotor exercise of a person larva stays constant all through a given day and is predictable all through larval growth, particularly throughout darkish phases, below which larvae display light-searching behaviors and elevated exercise.
 ELISA Kit) Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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DLR-GFAP-b-96T |
DL Develop |
96T |
EUR 858 |
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Description: A sandwich quantitative ELISA assay kit for detection of Bovine Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma or other biological fluids. |
 ELISA Kit) Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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DLR-GFAP-Hu-48T |
DL Develop |
48T |
EUR 574.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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DLR-GFAP-Hu-96T |
DL Develop |
96T |
EUR 745.2 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
 ELISA Kit) Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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DLR-GFAP-Mu-48T |
DL Develop |
48T |
EUR 586.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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DLR-GFAP-Mu-96T |
DL Develop |
96T |
EUR 762 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
 ELISA Kit) Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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DLR-GFAP-Ra-48T |
DL Develop |
48T |
EUR 609.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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DLR-GFAP-Ra-96T |
DL Develop |
96T |
EUR 793.2 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RD-GFAP-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 666 |
Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RD-GFAP-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 925.2 |
Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RD-GFAP-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 573.6 |
Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RD-GFAP-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 794.4 |
Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RD-GFAP-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 586.8 |
Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RD-GFAP-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 812.4 |
Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RD-GFAP-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 613.2 |
Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RD-GFAP-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 850.8 |
Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RDR-GFAP-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 696 |
Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RDR-GFAP-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 968.4 |
Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RDR-GFAP-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 600 |
Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RDR-GFAP-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 830.4 |
Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RDR-GFAP-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 613.2 |
Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RDR-GFAP-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 850.8 |
Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RDR-GFAP-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 640.8 |
Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit |
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RDR-GFAP-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 890.4 |
) Gabarapa Antibody (Zebrafish) |
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F53276-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 165 |
 GFAP Antibody |
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1-CSB-PA289722 |
Cusabio |
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Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100 |
GFAP Antibody |
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CSB-PA047116-100ul |
Cusabio |
100ul |
EUR 379.2 |
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Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200 |
GFAP Antibody |
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1-CSB-PA008787 |
Cusabio |
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Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000 |
GFAP Antibody |
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1-CSB-PA009369GA01HU |
Cusabio |
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Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
 gfap Antibody |
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1-CSB-PA009369LA01DIL |
Cusabio |
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Description: A polyclonal antibody against gfap. Recognizes gfap from Zebrafish, Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000 |
GFAP Antibody |
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1-CSB-PA009369LA01HU |
Cusabio |
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Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
 Gfap Antibody |
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1-CSB-PA009369LA01RA |
Cusabio |
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Description: A polyclonal antibody against Gfap. Recognizes Gfap from Rat, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000 |
 GFAP antibody |
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70R-35624 |
Fitzgerald |
100 ug |
EUR 392.4 |
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Description: Rabbit polyclonal GFAP antibody |
GFAP antibody |
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70R-GR030 |
Fitzgerald |
100 ug |
EUR 360 |
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Description: Affinity purified Rabbit polyclonal GFAP antibody |
GFAP antibody |
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70R-GR031 |
Fitzgerald |
100 ug |
EUR 360 |
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Description: Affinity purified Rabbit polyclonal GFAP antibody |
GFAP antibody |
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70R-9278 |
Fitzgerald |
50 ug |
EUR 560.4 |
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Description: Affinity purified rabbit polyclonal GFAP antibody |
GFAP Antibody |
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48610-100ul |
SAB |
100ul |
EUR 399.6 |
GFAP Antibody |
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48610-50ul |
SAB |
50ul |
EUR 286.8 |
GFAP Antibody |
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32033-100ul |
SAB |
100ul |
EUR 302.4 |
GFAP Antibody |
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42704-100ul |
SAB |
100ul |
EUR 302.4 |
GFAP antibody |
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70R-10640 |
Fitzgerald |
200 ul |
EUR 516 |
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Description: Affinity purified Chicken polyclonal GFAP antibody |
GFAP antibody |
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70R-10675 |
Fitzgerald |
1 ml |
EUR 447.6 |
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Description: Rabbit polyclonal GFAP antibody |
GFAP antibody |
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70R-17460 |
Fitzgerald |
50 ul |
EUR 522 |
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Description: Rabbit polyclonal GFAP antibody |
GFAP antibody |
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70R-13832 |
Fitzgerald |
100 ug |
EUR 366 |
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Description: Affinity purified Rabbit polyclonal GFAP antibody |
GFAP antibody |
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20R-2605 |
Fitzgerald |
100 uL |
EUR 639.6 |
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Description: Guinea Pig polyclonal GFAP antibody |
GFAP antibody |
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20R-2732 |
Fitzgerald |
50 ug |
EUR 337.2 |
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Description: Rabbit polyclonal GFAP antibody |
GFAP antibody |
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20R-2855 |
Fitzgerald |
100 ul |
EUR 418.8 |
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Description: Rabbit polyclonal GFAP antibody |
GFAP antibody |
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20R-2856 |
Fitzgerald |
100 ul |
EUR 418.8 |
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Description: Chicken polyclonal GFAP antibody |
GFAP antibody |
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20R-GR004 |
Fitzgerald |
500 ul |
EUR 535.2 |
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Description: Rabbit polyclonal GFAP antibody |
GFAP Antibody |
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21485-100ul |
SAB |
100ul |
EUR 302.4 |
GFAP Antibody |
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21485-50ul |
SAB |
50ul |
EUR 224.4 |
GFAP Antibody |
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35350-100ul |
SAB |
100ul |
EUR 468 |
GFAP antibody |
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20C-CR8013R |
Fitzgerald |
400 ul |
EUR 138 |
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Description: Rabbit polyclonal GFAP antibody |
GFAP antibody |
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10R-7859 |
Fitzgerald |
100 ug |
EUR 366 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-8068 |
Fitzgerald |
100 ug |
EUR 489.6 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-G105a |
Fitzgerald |
50 ug |
EUR 534 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-2253 |
Fitzgerald |
100 ul |
EUR 483.6 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-2384 |
Fitzgerald |
5 mL |
EUR 318 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-4205 |
Fitzgerald |
100 ul |
EUR 829.2 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-4206 |
Fitzgerald |
100 ul |
EUR 829.2 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-4208 |
Fitzgerald |
100 ul |
EUR 829.2 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-4209 |
Fitzgerald |
100 ul |
EUR 829.2 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-4210 |
Fitzgerald |
100 ul |
EUR 829.2 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-4212 |
Fitzgerald |
100 ul |
EUR 829.2 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-4213 |
Fitzgerald |
100 ul |
EUR 829.2 |
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Description: Mouse monoclonal GFAP antibody |
GFAP antibody |
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10R-4214 |
Fitzgerald |
100 ul |
EUR 829.2 |
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Description: Mouse monoclonal GFAP antibody |
GFAP Antibody |
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RQ6032 |
NSJ Bioreagents |
100 ug |
EUR 419 |
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Description: Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell's cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. GFAP is necessary for many critical roles in the CNS. What's more, GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication. |
GFAP Antibody |
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CSB-PA047116- |
Cusabio |
each |
EUR 402 |
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Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200 |
GFAP Antibody |
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3206-200 |
Biovision |
each |
EUR 418.8 |
GFAP Antibody |
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3206-30T |
Biovision |
each |
EUR 175.2 |
 Antibody) Nicalin Homolog (Zebrafish) Antibody |
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20-abx114104 |
Abbexa |
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 Zebrafish beta Actin Antibody |
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F52293-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 165 |
) Pou5f1 Antibody OCT4 (Zebrafish) |
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F52574-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 165 |
) Beclin-1 Antibody (Zebrafish) |
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F53282-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 165 |
 GFAP Conjugated Antibody |
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C21485 |
SAB |
100ul |
EUR 476.4 |
 GFAP Polyclonal Antibody |
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ABP54511-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
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Description: A polyclonal antibody for detection of GFAP from Human. This GFAP antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GFAP around the non-phosphorylation site of S38 |
GFAP Polyclonal Antibody |
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ABP54511-01ml |
Abbkine |
0.1ml |
EUR 346.8 |
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Description: A polyclonal antibody for detection of GFAP from Human. This GFAP antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GFAP around the non-phosphorylation site of S38 |
GFAP Polyclonal Antibody |
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ABP54511-02ml |
Abbkine |
0.2ml |
EUR 496.8 |
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Description: A polyclonal antibody for detection of GFAP from Human. This GFAP antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GFAP around the non-phosphorylation site of S38 |
The larvae’s response to startle-stimuli was discovered to be unpredictable, with no correlation discovered between response power and locomotor exercise. Moreover, locomotor exercise was not related to physiological or morphological options of a larva (resting coronary heart fee, physique size, dimension of the swim bladder). Total, our findings spotlight the areas of intra-individual consistency, which could possibly be used to enhance the sensitivity of assays utilizing zebrafish locomotor exercise as an endpoint.
