On the Observation of Wild Zebrafish (Danio rerio) in India.

On the Observation of Wild Zebrafish (Danio rerio) in India.

Zebrafish is without doubt one of the world’s most generally used laboratory species, and it’s utilized to reply vital analysis questions in disparate fields reminiscent of biomedicine, genetics, developmental biology, pharmacology, toxicology, physiology, and evolution. Regardless of their reputation, little or no is understood concerning the biology of zebrafish of their pure habitat.

This may increasingly, partially, be because of the difficulties related to endeavor area journeys to the distant areas of northern India, Nepal, and Bangladesh, which is the pure distribution vary of zebrafish. Right here, we current a area report describing a latest journey the place we, along with native collaborators, visited a number of rivers in West Bengal, India, to watch wild zebrafish and their habitat. We current an outline of our observations on the biology of untamed zebrafish, and the nice variability of the totally different environments the place they have been discovered.

We additionally embrace knowledge collected on water chemistry parameters at 12 zebrafish websites, and weight knowledge and pictures of fish from these websites. We current in depth underwater movies of untamed zebrafish and images of the websites, together with video footage of courtship conduct. We present that the breeding interval of untamed zebrafish could be prolonged from the earlier file of April-August to April-October. As well as, we offer sensible recommendation for future zebrafish expeditions to this rural and inaccessible space. The objectives of this text are to shed some mild on the ecology of untamed zebrafish, and to facilitate scientists of their future analysis journeys. We hope that by observing zebrafish within the wild, we are able to enhance our understanding of the pure ecology of this vital mannequin organism.

Use of Zebrafish in Drug Discovery Toxicology.

Unpredicted human security occasions in scientific trials for brand new medicine are expensive when it comes to human well being and cash. The drug discovery business makes an attempt to attenuate these occasions with diligent preclinical security testing. Present normal practices are good at stopping poisonous compounds from being examined within the clinic; nonetheless, false unfavorable preclinical toxicity outcomes are nonetheless a actuality. Continuous enchancment have to be pursued within the preclinical realm. Increased high quality therapies could be introduced ahead with extra details about potential toxicities and related mechanisms.
The zebrafish mannequin is a bridge between in vitro assays and mammalian in vivo research. This mannequin is highly effective in its breadth of utility and tractability for analysis. Prior to now 20 years our understanding of illness biology and drug toxicity has grown considerably owing to hundreds of research on this tiny vertebrate. This overview summarizes challenges and strengths of the mannequin, discusses the 3Rs worth that it could ship, highlights translatable and untranslatable biology, and brings collectively experiences from latest research with zebrafish specializing in new drug discovery toxicology.
The priority about DNA injury has directed efforts towards evaluating the genotoxic potential of bodily and chemical brokers. For the reason that extent of DNA injury can be associated to the capability of the organism in repairing the DNA, the advance of toxicological research on this space depends upon the characterization of the DNA restore mechanisms within the accessible fashions. The mobile zebrafish fashions, for instance, exchange mammalian cells to reply ecologically related questions on aquatic toxicology.
So, the intention of the current research was to characterize the nucleotide excision restore (NER) and photoreactivation (PER) in two mobile fashions of Danio rerio liver, major hepatocytes and ZF-L (Zebrafish Liver) cell line. We carried out kinetic research of the DNA injury ranges after publicity to six.eight J/m2 UVC utilizing the T4-PDG modified Comet Assay, and decided the expression ranges of vital genes concerned in NER, PER and base excision restore utilizing RT-qPCR.
On the Observation of Wild Zebrafish (Danio rerio) in India.

Emergence of constant intra-individual locomotor patterns throughout zebrafish growth.

The evaluation of larval zebrafish locomotor conduct has emerged as a strong indicator of perturbations within the nervous system and is utilized in many fields of analysis, together with neuroscience, toxicology and drug discovery. The conduct of larval zebrafish nonetheless, is extremely variable, leading to the usage of giant numbers of animals and the lack to detect small results.
On this research, we analyzed whether or not particular person locomotor conduct is secure over growth and whether or not behavioral parameters correlate with physiological and morphological options, with the intention of higher understanding the variability and predictability of larval locomotor conduct. Our outcomes reveal that locomotor exercise of a person larva stays constant all through a given day and is predictable all through larval growth, particularly throughout darkish phases, below which larvae display light-searching behaviors and elevated exercise.

Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

DLR-GFAP-b-96T 96T
EUR 858
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma or other biological fluids.

Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

DLR-GFAP-Hu-48T 48T
EUR 574.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

DLR-GFAP-Hu-96T 96T
EUR 745.2
Description: A sandwich quantitative ELISA assay kit for detection of Human Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

DLR-GFAP-Mu-48T 48T
EUR 586.8
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

DLR-GFAP-Mu-96T 96T
EUR 762
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

DLR-GFAP-Ra-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

DLR-GFAP-Ra-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glial Fibrillary Acidic Protein (GFAP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RD-GFAP-b-48Tests 48 Tests
EUR 666

Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RD-GFAP-b-96Tests 96 Tests
EUR 925.2

Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RD-GFAP-Hu-48Tests 48 Tests
EUR 573.6

Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RD-GFAP-Hu-96Tests 96 Tests
EUR 794.4

Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RD-GFAP-Mu-48Tests 48 Tests
EUR 586.8

Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RD-GFAP-Mu-96Tests 96 Tests
EUR 812.4

Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RD-GFAP-Ra-48Tests 48 Tests
EUR 613.2

Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RD-GFAP-Ra-96Tests 96 Tests
EUR 850.8

Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RDR-GFAP-b-48Tests 48 Tests
EUR 696

Bovine Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RDR-GFAP-b-96Tests 96 Tests
EUR 968.4

Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RDR-GFAP-Hu-48Tests 48 Tests
EUR 600

Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RDR-GFAP-Hu-96Tests 96 Tests
EUR 830.4

Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RDR-GFAP-Mu-48Tests 48 Tests
EUR 613.2

Mouse Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RDR-GFAP-Mu-96Tests 96 Tests
EUR 850.8

Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RDR-GFAP-Ra-48Tests 48 Tests
EUR 640.8

Rat Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

RDR-GFAP-Ra-96Tests 96 Tests
EUR 890.4

Zebrafish Patched Antibody

R35480-100UG 100 ug
EUR 399

Zebrafish Thrb Antibody

R33481-100UG 100 ug
EUR 399

Pou3f3a Antibody (Zebrafish)

F52241-0.08ML 0.08 ml
EUR 165

Pou3f3a Antibody (Zebrafish)

F52241-0.4ML 0.4 ml
EUR 379

Zebrafish Atf4 Antibody

F52258-0.08ML 0.08 ml
EUR 165

Zebrafish Atf4 Antibody

F52258-0.4ML 0.4 ml
EUR 379

Zebrafish Pax2a Antibody

F52269-0.08ML 0.08 ml
EUR 165

Zebrafish Pax2a Antibody

F52269-0.4ML 0.4 ml
EUR 379

S1pr1 Antibody (Zebrafish)

F52393-0.08ML 0.08 ml
EUR 165

Gapdh Antibody (Zebrafish)

F52573-0.08ML 0.08 ml
EUR 165

Zebrafish Gapdh Antibody

F52573-0.4ML 0.4 ml
EUR 379

Ada Antibody (Zebrafish)

F52652-0.08ML 0.08 ml
EUR 165

Zebrafish Ada Antibody

F52652-0.4ML 0.4 ml
EUR 379

Zebrafish Map1lc3a Antibody

F53023-0.08ML 0.08 ml
EUR 165

Zebrafish Map1lc3a Antibody

F53023-0.4ML 0.4 ml
EUR 379

Zebrafish LC3A Antibody

F53024-0.08ML 0.08 ml
EUR 165

Zebrafish LC3A Antibody

F53024-0.4ML 0.4 ml
EUR 379

Atg5 Antibody (Zebrafish)

F53143-0.08ML 0.08 ml
EUR 165

Gabarapa Antibody (Zebrafish)

F53276-0.08ML 0.08 ml
EUR 165

Zebrafish LC3B Antibody

F53289-0.08ML 0.08 ml
EUR 165

Zebrafish LC3B Antibody

F53289-0.4ML 0.4 ml
EUR 379

Atg5 Antibody (Zebrafish)

F53381-0.08ML 0.08 ml
EUR 165

Atg5 Antibody (Zebrafish)

F53381-0.4ML 0.4 ml
EUR 379

ak2 Antibody (Zebrafish)

F54506-0.05ML 0.05 ml
EUR 165

ak2 Antibody (Zebrafish)

F54506-0.2ML 0.2 ml
EUR 379

GFAP Antibody

1-CSB-PA289722
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

GFAP Antibody

CSB-PA047116-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

GFAP Antibody

1-CSB-PA008787
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000

GFAP Antibody

1-CSB-PA009369GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

gfap Antibody

1-CSB-PA009369LA01DIL
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against gfap. Recognizes gfap from Zebrafish, Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000

GFAP Antibody

1-CSB-PA009369LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Gfap Antibody

1-CSB-PA009369LA01RA
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Gfap. Recognizes Gfap from Rat, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000

GFAP Antibody

DF6040 200ul
EUR 420

GFAP Antibody

AF6166 200ul
EUR 420

GFAP antibody

70R-35624 100 ug
EUR 392.4
Description: Rabbit polyclonal GFAP antibody

GFAP antibody

70R-GR030 100 ug
EUR 360
Description: Affinity purified Rabbit polyclonal GFAP antibody

GFAP antibody

70R-GR031 100 ug
EUR 360
Description: Affinity purified Rabbit polyclonal GFAP antibody

GFAP antibody

70R-9278 50 ug
EUR 560.4
Description: Affinity purified rabbit polyclonal GFAP antibody

GFAP Antibody

48610-100ul 100ul
EUR 399.6

GFAP Antibody

48610-50ul 50ul
EUR 286.8

GFAP Antibody

32033-100ul 100ul
EUR 302.4

GFAP Antibody

42704-100ul 100ul
EUR 302.4

GFAP antibody

70R-10640 200 ul
EUR 516
Description: Affinity purified Chicken polyclonal GFAP antibody

GFAP antibody

70R-10675 1 ml
EUR 447.6
Description: Rabbit polyclonal GFAP antibody

GFAP antibody

70R-17460 50 ul
EUR 522
Description: Rabbit polyclonal GFAP antibody

GFAP antibody

70R-13832 100 ug
EUR 366
Description: Affinity purified Rabbit polyclonal GFAP antibody

GFAP antibody

20R-2605 100 uL
EUR 639.6
Description: Guinea Pig polyclonal GFAP antibody

GFAP antibody

20R-2732 50 ug
EUR 337.2
Description: Rabbit polyclonal GFAP antibody

GFAP antibody

20R-2855 100 ul
EUR 418.8
Description: Rabbit polyclonal GFAP antibody

GFAP antibody

20R-2856 100 ul
EUR 418.8
Description: Chicken polyclonal GFAP antibody

GFAP antibody

20R-GR004 500 ul
EUR 535.2
Description: Rabbit polyclonal GFAP antibody

GFAP Antibody

21485-100ul 100ul
EUR 302.4

GFAP Antibody

21485-50ul 50ul
EUR 224.4

GFAP Antibody

35350-100ul 100ul
EUR 468

GFAP antibody

20C-CR8013R 400 ul
EUR 138
Description: Rabbit polyclonal GFAP antibody

GFAP antibody

10R-7859 100 ug
EUR 366
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-8068 100 ug
EUR 489.6
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-G105a 50 ug
EUR 534
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-2253 100 ul
EUR 483.6
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-2384 5 mL
EUR 318
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-4205 100 ul
EUR 829.2
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-4206 100 ul
EUR 829.2
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-4208 100 ul
EUR 829.2
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-4209 100 ul
EUR 829.2
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-4210 100 ul
EUR 829.2
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-4212 100 ul
EUR 829.2
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-4213 100 ul
EUR 829.2
Description: Mouse monoclonal GFAP antibody

GFAP antibody

10R-4214 100 ul
EUR 829.2
Description: Mouse monoclonal GFAP antibody

GFAP Antibody

ABF6166 100 ug
EUR 525.6

GFAP Antibody

ABD6040 100 ug
EUR 525.6

GFAP Antibody

BF0345 200ul
EUR 540

GFAP Antibody

R30340 100 ug
EUR 419

GFAP Antibody

R31508 100 ug
EUR 419

GFAP Antibody

R33452-100UG 100 ug
EUR 399

GFAP Antibody

RQ6032 100 ug
EUR 419
Description: Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell's cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. GFAP is necessary for many critical roles in the CNS. What's more, GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication.

GFAP Antibody

CSB-PA047116- each
EUR 402
Description: A polyclonal antibody against GFAP. Recognizes GFAP from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

GFAP Antibody

F47814-0.4ML 0.4 ml
EUR 379

GFAP Antibody

3206-200 each
EUR 418.8

GFAP Antibody

3206-30T each
EUR 175.2

Nicalin Homolog (Zebrafish) Antibody

20-abx114104
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Anti-Tfap2a (zebrafish) antibody

STJ71254 100 µg
EUR 312

Anti-Ascl1a (zebrafish) antibody

STJ71255 100 µg
EUR 312

Anti-Hand2 (zebrafish) antibody

STJ71256 100 µg
EUR 312

Anti-Notch1a (zebrafish) antibody

STJ71260 100 µg
EUR 312

Anti-Notch1b (zebrafish) antibody

STJ71261 100 µg
EUR 312

Anti-Six3b (zebrafish) antibody

STJ71263 100 µg
EUR 312

Anti-Sox9a (zebrafish) antibody

STJ71264 100 µg
EUR 312

Anti-Sox9b (zebrafish) antibody

STJ71265 100 µg
EUR 312

Anti-Thrb (zebrafish) antibody

STJ71268 100 µg
EUR 430.8

Anti-Timp2 (zebrafish) antibody

STJ71270 100 µg
EUR 312

Anti-Timp2L (zebrafish) antibody

STJ71271 100 µg
EUR 312

Anti-Atoh7 (zebrafish) antibody

STJ71272 100 µg
EUR 312

Anti-Ptf1a (zebrafish) antibody

STJ71274 100 µg
EUR 312

Anti-Cxcr4b (zebrafish) antibody

STJ71293 100 µg
EUR 312

Anti-Her6 (zebrafish) antibody

STJ71294 100 µg
EUR 312

Zebrafish beta Actin Antibody

F52293-0.08ML 0.08 ml
EUR 165

Zebrafish beta Actin Antibody

F52293-0.4ML 0.4 ml
EUR 379

Pou5f1 Antibody OCT4 (Zebrafish)

F52574-0.08ML 0.08 ml
EUR 165

Beclin-1 Antibody (Zebrafish)

F53282-0.08ML 0.08 ml
EUR 165

Zebrafish Beclin-1 Antibody

F53282-0.4ML 0.4 ml
EUR 379

GFAP

CH22102 100 ul
EUR 490.8

GFAP

CH23011 200 ul
EUR 553.2

GFAP

MO15052 100 ul
EUR 528

GFAP

MO22136 100 ul
EUR 522

GFAP

MO45001 100 ug
EUR 678

GFAP

RA22101 100 ul
EUR 490.8

GFAP

SP15101 100 ug
EUR 646.8

GFAP Conjugated Antibody

C21485 100ul
EUR 476.4

GFAP Polyclonal Antibody

ABP54511-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GFAP from Human. This GFAP antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GFAP around the non-phosphorylation site of S38

GFAP Polyclonal Antibody

ABP54511-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GFAP from Human. This GFAP antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GFAP around the non-phosphorylation site of S38

GFAP Polyclonal Antibody

ABP54511-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GFAP from Human. This GFAP antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human GFAP around the non-phosphorylation site of S38
The larvae’s response to startle-stimuli was discovered to be unpredictable, with no correlation discovered between response power and locomotor exercise. Moreover, locomotor exercise was not related to physiological or morphological options of a larva (resting coronary heart fee, physique size, dimension of the swim bladder). Total, our findings spotlight the areas of intra-individual consistency, which could possibly be used to enhance the sensitivity of assays utilizing zebrafish locomotor exercise as an endpoint.

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