Casting a wide net: use of diverse model organisms to advance toxicology.

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Casting a wide net: use of diverse model organisms to advance toxicology.

Toxicology – the research of how chemical compounds work together with organic techniques – has clear relevance to human well being and illness. Persistent publicity to pure and artificial chemical compounds is an unavoidable a part of residing on our planet; but, we perceive little or no concerning the results of publicity to the overwhelming majority of chemical compounds. Whereas epidemiological research can present robust statistical inference linking chemical publicity to illness, analysis in mannequin techniques is crucial to elucidate the mechanisms of motion and to foretell outcomes.

Most analysis in toxicology makes use of a handful of mammalian fashions that symbolize a number of distinct branches of the evolutionary tree. This slender focus constrains the understanding of chemical-induced illness processes and techniques which have developed in response to exposures. We advocate for casting a wider internet in environmental toxicology analysis to make the most of various mannequin techniques, together with zebrafish, and carry out extra mechanistic research of mobile responses to chemical exposures to shift the notion of toxicology as an utilized science to that of a primary science. This more-inclusive perspective will enrich the sector and may stay central to analysis on chemical-induced illness.

Traditionally, analysis in toxicology has utilized non-human mammalian species, notably rats and mice, to check in vivo the results of poisonous publicity on physiology and conduct. Nevertheless, moral issues and the overwhelming enhance within the variety of chemical compounds to be screened has led to a shift away from in vivo work. The decline in in vivo experimentation has been accompanied by a rise in various strategies for detecting and predicting detrimental results: in vitro experimentation and in silico modeling.

But, these new methodologies can’t substitute the necessity for in vivo work on animal physiology and conduct. The event of recent, non-mammalian mannequin techniques exhibits nice promise in restoring our skill to make use of behavioral endpoints in toxicological testing. Of those techniques, the zebrafish, Danio rerio, is the mannequin organism for which we’re accumulating sufficient information in vivo, in vitro, and in silico to allow us to develop a complete, highthroughput toxicology screening system.

Developmental toxicity of fipronil in early improvement of zebrafish (Danio rerio) larvae: Disrupted vascular formation with angiogenic failure and inhibited neurogenesis.

Fipronil has been extensively utilized in agriculture to forestall aggressive bugs from damaging agricultural merchandise. Fipronil residues flow into within the setting they usually have been detected in non-targeted organisms in aquatic environments. To check the impact of fipronil toxicity on environmental well being, 6 h put up fertilization (hpf) zebrafish embryos have been handled with fipronil for 72 h. LC50 worth was obtained by making use of various concentrations of fipronil to zebrafish embryos for 72 h.

As zebrafish embryos are helpful vertebrate fashions for finding out developmental and genetic findings in toxicology analysis, they have been uncovered to fipronil to check detailed elucidating mechanisms with hazardous finish factors of toxicity. Cell cycle arrest-related apoptosis supported pathological alterations, equivalent to elevated mortality, shortened physique size, and lowered hatchability.

Moreover, noticed coronary heart defects, together with edema and irregular heartbeat have been triggered because of irregular blood circulation. In transgenic zebrafish fashions (fli1:eGFP and olig2:dsRED), disrupted blood vessel formations have been indicated by eGFP+ endothelial cells. Furthermore, neurogenic defects have been noticed by finding out dsRED+ motor neurons and oligodendrocytes. This research demonstrates fipronil accumulation in aquatic setting and its skill to impair important processes, equivalent to angiogenesis and neurogenesis throughout early developmental stage of zebrafish, together with normal developmental toxicity.

Casting a wide net: use of diverse model organisms to advance toxicology.

Twenty years of transcriptomics, 17alpha-ethinylestradiol, and fish.

In aquatic toxicology, maybe no pharmaceutical has been investigated extra intensely than 17alpha-ethinylestradiol (EE2), the lively ingredient of the contraception capsule. On the flip of the century, the fields of comparative endocrinology and endocrine disruption analysis witnessed the emergence of omics applied sciences, which have been quickly tailored to characterize potential hazards related to exposures to environmental estrogens, equivalent to EE2.
Since then, vital advances have been made by the scientific neighborhood, and in consequence, a lot has been realized about estrogen receptor signaling in fish from environmental xenoestrogens. Vitellogenin, the egg yolk precursor protein, was recognized as a significant estrogen-responsive gene, establishing itself because the premier biomarker for estrogenic exposures. Omics research have recognized a plethora of estrogen responsive genes, contributing to a wealth of data on estrogen-mediated regulatory networks in teleosts.
There have been ∼40 research that report on transcriptome responses to EE2 in quite a lot of fish species (e.g., zebrafish, fathead minnows, rainbow trout, pipefish, mummichog, stickleback, cod, and others). Information on the liver and testis transcriptome dominate within the literature and have been the topic of many EE2 research, but there stay information gaps for different tissues, such because the spleen, kidney, and pituitary. Inter-laboratory genomics research have revealed transcriptional networks altered by EE2 remedy within the liver; networks associated to amino acid activation and protein folding are elevated by EE2 whereas these associated to xenobiotic metabolism, immune system, circulation, and triglyceride storage are suppressed.

Recombinant Zebrafish GH, Mutant Protein, Untagged, E.coli-50ug
QP11965-50ug 50ug
EUR 201

Zebrafish Growth Hormone Mutant Protein
20-abx260619
  • EUR 2903.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.

Recombinant Zebrafish Growth Hormone Mutant
7-00574 5µg Ask for price

Recombinant Zebrafish Growth Hormone Mutant
7-00575 50µg Ask for price

Recombinant Zebrafish Growth Hormone Mutant
7-00576 1mg Ask for price

Recombinant Zebrafish GH Protein, Untagged, E.coli-10ug
QP10635-10ug 10ug
EUR 155

Recombinant Zebrafish GH Protein, Untagged, E.coli-1mg
QP10635-1mg 1mg
EUR 2358

Recombinant Zebrafish GH Protein, Untagged, E.coli-50ug
QP10635-50ug 50ug
EUR 201

Bovine Growth Hormone (GH) ELISA Kit
DLR-GH-b-48T 48T
EUR 547
  • Should the Bovine Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Growth Hormone (GH) in samples from serum, plasma, tissue homogenates or other biological fluids.

Bovine Growth Hormone (GH) ELISA Kit
DLR-GH-b-96T 96T
EUR 715
  • Should the Bovine Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Growth Hormone (GH) in samples from serum, plasma, tissue homogenates or other biological fluids.

Canine Growth Hormone (GH) ELISA Kit
DLR-GH-c-48T 48T
EUR 527
  • Should the Canine Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Canine Growth Hormone (GH) ELISA Kit
DLR-GH-c-96T 96T
EUR 688
  • Should the Canine Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Chicken Growth Hormone (GH) ELISA Kit
DLR-GH-Ch-48T 48T
EUR 508
  • Should the Chicken Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Chicken Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Chicken Growth Hormone (GH) ELISA Kit
DLR-GH-Ch-96T 96T
EUR 661
  • Should the Chicken Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Chicken Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Equine Growth Hormone (GH) ELISA Kit
DLR-GH-Eq-48T 48T
EUR 556
  • Should the Equine Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Equine Growth Hormone (GH) ELISA Kit
DLR-GH-Eq-96T 96T
EUR 728
  • Should the Equine Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Equine Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Human Growth Hormone (GH) ELISA Kit
DLR-GH-Hu-48T 48T
EUR 381
  • Should the Human Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Growth Hormone (GH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Growth Hormone (GH) ELISA Kit
DLR-GH-Hu-96T 96T
EUR 487
  • Should the Human Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Growth Hormone (GH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Growth Hormone (GH) ELISA Kit
DLR-GH-Mu-48T 48T
EUR 489
  • Should the Mouse Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Growth Hormone (GH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Growth Hormone (GH) ELISA Kit
DLR-GH-Mu-96T 96T
EUR 635
  • Should the Mouse Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Growth Hormone (GH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Growth Hormone (GH) ELISA Kit
DLR-GH-p-48T 48T
EUR 547
  • Should the Porcine Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Porcine Growth Hormone (GH) ELISA Kit
DLR-GH-p-96T 96T
EUR 715
  • Should the Porcine Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Rat Growth Hormone (GH) ELISA Kit
DLR-GH-Ra-48T 48T
EUR 508
  • Should the Rat Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Rat Growth Hormone (GH) ELISA Kit
DLR-GH-Ra-96T 96T
EUR 661
  • Should the Rat Growth Hormone (GH) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Growth Hormone (GH) in samples from serum, plasma or other biological fluids.

Bovine Growth Hormone (GH) ELISA Kit
RDR-GH-b-48Tests 48 Tests
EUR 580

Bovine Growth Hormone (GH) ELISA Kit
RDR-GH-b-96Tests 96 Tests
EUR 807

Canine Growth Hormone (GH) ELISA Kit
RDR-GH-c-48Tests 48 Tests
EUR 557

Canine Growth Hormone (GH) ELISA Kit
RDR-GH-c-96Tests 96 Tests
EUR 774

Chicken Growth Hormone (GH) ELISA Kit
RDR-GH-Ch-48Tests 48 Tests
EUR 534

Chicken Growth Hormone (GH) ELISA Kit
RDR-GH-Ch-96Tests 96 Tests
EUR 742

Equine Growth Hormone (GH) ELISA Kit
RDR-GH-Eq-48Tests 48 Tests
EUR 591

Equine Growth Hormone (GH) ELISA Kit
RDR-GH-Eq-96Tests 96 Tests
EUR 823

Human Growth Hormone (GH) ELISA Kit
RDR-GH-Hu-48Tests 48 Tests
EUR 384

Human Growth Hormone (GH) ELISA Kit
RDR-GH-Hu-96Tests 96 Tests
EUR 527

Mouse Growth Hormone (GH) ELISA Kit
RDR-GH-Mu-48Tests 48 Tests
EUR 511

Mouse Growth Hormone (GH) ELISA Kit
RDR-GH-Mu-96Tests 96 Tests
EUR 709

Porcine Growth Hormone (GH) ELISA Kit
RDR-GH-p-48Tests 48 Tests
EUR 580

Porcine Growth Hormone (GH) ELISA Kit
RDR-GH-p-96Tests 96 Tests
EUR 807

Rat Growth Hormone (GH) ELISA Kit
RDR-GH-Ra-48Tests 48 Tests
EUR 534

Rat Growth Hormone (GH) ELISA Kit
RDR-GH-Ra-96Tests 96 Tests
EUR 742

Bovine Growth Hormone (GH) ELISA Kit
RD-GH-b-48Tests 48 Tests
EUR 555

Bovine Growth Hormone (GH) ELISA Kit
RD-GH-b-96Tests 96 Tests
EUR 771

Canine Growth Hormone (GH) ELISA Kit
RD-GH-c-48Tests 48 Tests
EUR 533

Canine Growth Hormone (GH) ELISA Kit
RD-GH-c-96Tests 96 Tests
EUR 740

Chicken Growth Hormone (GH) ELISA Kit
RD-GH-Ch-48Tests 48 Tests
EUR 511

Chicken Growth Hormone (GH) ELISA Kit
RD-GH-Ch-96Tests 96 Tests
EUR 709

Equine Growth Hormone (GH) ELISA Kit
RD-GH-Eq-48Tests 48 Tests
EUR 566

Equine Growth Hormone (GH) ELISA Kit
RD-GH-Eq-96Tests 96 Tests
EUR 787

Human Growth Hormone (GH) ELISA Kit
RD-GH-Hu-48Tests 48 Tests
EUR 368

Human Growth Hormone (GH) ELISA Kit
RD-GH-Hu-96Tests 96 Tests
EUR 504

Mouse Growth Hormone (GH) ELISA Kit
RD-GH-Mu-48Tests 48 Tests
EUR 489

Mouse Growth Hormone (GH) ELISA Kit
RD-GH-Mu-96Tests 96 Tests
EUR 677

Porcine Growth Hormone (GH) ELISA Kit
RD-GH-p-48Tests 48 Tests
EUR 555

Porcine Growth Hormone (GH) ELISA Kit
RD-GH-p-96Tests 96 Tests
EUR 771

Rat Growth Hormone (GH) ELISA Kit
RD-GH-Ra-48Tests 48 Tests
EUR 511

Rat Growth Hormone (GH) ELISA Kit
RD-GH-Ra-96Tests 96 Tests
EUR 709

p53 Mutant Protein
20-abx263550
  • EUR 829.00
  • EUR 411.00
  • EUR 537.00
  • 10 ug
  • 2 µg
  • 5 ug
  • Shipped within 5-10 working days.

Resistin Mutant Protein
20-abx263076
  • EUR 328.00
  • EUR 8179.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Zebrafish Growth Hormone Protein
20-abx260620
  • EUR 2903.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.

Zebrafish Talin (TLN) Protein
20-abx655190
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Chikungunya E1 Mutant Protein
abx160006-1mg 1 mg
EUR 1442
  • Shipped within 5-10 working days.

Zebrafish Cathepsin L (CTSL) Protein
20-abx652813
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Zebrafish Galactosidase Beta (GLb) Protein
20-abx653494
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Zebrafish Interleukin 12B (IL12B) Protein
20-abx653962
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Zebrafish Interleukin 15 (IL15) Protein
20-abx653977
  • EUR 899.00
  • EUR 328.00
  • EUR 2931.00
  • EUR 1094.00
  • EUR 634.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Ovine Recombinant (E.Coli) Purified Leptin Quadruple mutant mutant Antagonist Protein
LEP26-QM-10 10 ug
EUR 164

Ovine Recombinant (E.Coli) Purified Leptin Quadruple mutant mutant Antagonist Protein
LEP26-QM-50 50 ug
EUR 408

Cytomegalovirus gH (CMV gH) Antibody
abx415688-01mg 0.1 mg
EUR 648
  • Shipped within 1 week.

Sheep Prolactin Antagonist, Mutant Protein
20-abx263509
  • EUR 230.00
  • EUR 1790.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.

Chikungunya Mutant (A226V) E1 Protein
20-abx262346
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Resistin Mutant Human Recombinant Protein
PROTQ9HD89-2 Regular: 10ug
EUR 317
Description: 9.9 kDa protein containing 93 amino acid residues, produced in E.coli. Mutant-Resistin has had a Cysteine residue mutated to prevent dimerization and possibly acts as an antagonist.

Mutant p53 Antibody
49815-100ul 100ul
EUR 333

Mutant p53 Antibody
49815-50ul 50ul
EUR 239

Mutant EGFR inhibitor
B1103-10 10 mg
EUR 954
Description: Mutant EGFR inhibitor is a selective and potent Mutated EGFR inhibitor(L858R activating mutant, the Exonl9 deletion activating mutant and the T790M resistance mutant).

Mutant EGFR inhibitor
B1103-100 100 mg
EUR 3850
Description: Mutant EGFR inhibitor is a selective and potent Mutated EGFR inhibitor(L858R activating mutant, the Exonl9 deletion activating mutant and the T790M resistance mutant).

Mutant EGFR inhibitor
B1103-5 5 mg
EUR 683
Description: Mutant EGFR inhibitor is a selective and potent Mutated EGFR inhibitor(L858R activating mutant, the Exonl9 deletion activating mutant and the T790M resistance mutant).

Mutant EGFR inhibitor
B1103-5.1 10 mM (in 1mL DMSO)
EUR 775
Description: Mutant EGFR inhibitor is a selective and potent Mutated EGFR inhibitor(L858R activating mutant, the Exonl9 deletion activating mutant and the T790M resistance mutant).

Mutant EGFR inhibitor
B1103-50 50 mg
EUR 2764
Description: Mutant EGFR inhibitor is a selective and potent Mutated EGFR inhibitor(L858R activating mutant, the Exonl9 deletion activating mutant and the T790M resistance mutant).

EGFR mutant Antibody
abx015844-100ul 100 ul
EUR 411
  • Shipped within 5-10 working days.

EGFR mutant Antibody
abx015845-100ug 100 ug
EUR 411
  • Shipped within 5-10 working days.

Mutant IDH1 inhibitor
HY-13972 50mg
EUR 2116

Mutant EGFR inhibitor
HY-13984 5mg
EUR 533

M51Super8xFOPFlash (TOPFlash mutant)
PVT10790 2 ug
EUR 301

TNF-a-Mutant Tumor Necrosis Factor-Alpha Mutant Human Recombinant Protein
PROTP01375-2 Regular: 50ug
EUR 317
Description: Tumor Necrosis Factor-a Variant Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 151 amino acids and having a molecular mass of 16598 Dalton. ;The TNF-alpha Variant is purified by standard chromatographic techniques.

Zebrafish Creatine Kinase, Muscle (CKM) Protein
20-abx653069
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Zebrafish Gonadotropin Releasing Hormone (GnRH) Protein
20-abx653622
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Recombinant Zebrafish Growth Hormone
7-00571 5µg Ask for price

Recombinant Zebrafish Growth Hormone
7-00572 50µg Ask for price

Recombinant Zebrafish Growth Hormone
7-00573 1mg Ask for price

Nicalin Homolog (Zebrafish) Antibody
20-abx114104
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Anti-Tfap2a (zebrafish) antibody
STJ71254 100 µg
EUR 260

Anti-Ascl1a (zebrafish) antibody
STJ71255 100 µg
EUR 260

Anti-Hand2 (zebrafish) antibody
STJ71256 100 µg
EUR 260

Anti-Notch1a (zebrafish) antibody
STJ71260 100 µg
EUR 260

Anti-Notch1b (zebrafish) antibody
STJ71261 100 µg
EUR 260

Anti-Six3b (zebrafish) antibody
STJ71263 100 µg
EUR 260

Anti-Sox9a (zebrafish) antibody
STJ71264 100 µg
EUR 260

Anti-Sox9b (zebrafish) antibody
STJ71265 100 µg
EUR 260
EE2-responsive networks in different tissues aren’t as comprehensively outlined which is a information hole as regulated networks are anticipated to be tissue-specific. On the horizon, omics research for estrogen-mediated results in fish embody: (1) Establishing conceptual frameworks for incorporating estrogen-responsive networks into environmental monitoring packages; (2) Leveraging in vitro and computational toxicology approaches to determine chemical compounds related to estrogen receptor-mediated results in fish (e.g., male vitellogenin manufacturing); (3) Discovering new tissue-specific estrogen receptor signaling pathways in fish; and (4) Creating quantitative adversarial final result pathway predictive fashions for estrogen signaling.
As we glance forward, analysis into EE2 over the previous a number of a long time can function a template for the array of hormones and endocrine lively substances but to be absolutely characterised or found.

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